Oligonucleotide immobilization on micropatterned streptavidin surfaces
نویسندگان
چکیده
منابع مشابه
Oligonucleotide immobilization on micropatterned streptavidin surfaces.
We describe a simple procedure for photolithographic patterning of streptavidin on silicon substrates. Long wavelength UV (365 nm) light was used to direct the covalent attachment of photoactivatable biotin onto silylated silicon wafers. Fluorescently labeled streptavidin was found to bind only in areas exposed to the light. We used this procedure to selectively pattern streptavidin inside micr...
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Experimental control over cellular polarity in a neuronal network is a promising tool to study synapse formation and network behavior. We aimed to exploit a mechanism described by Stenger et al. [J. Neurosci. Methods 82 (1998) 167] to manipulate the direction of axonal versus dendritic outgrowth on a micropattern. The group had used laser ablation to create patterns of aminated silanes for cell...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2000
ISSN: 1362-4962
DOI: 10.1093/nar/28.8.e33